Quick Microscope Education
Glossary
Part of searching for the right scope is understanding the terminology and
anatomy of a microscope. Quickly read through this section and you should be
able to make a best choice for your needs.
Eyepiece: The lens closest to your eye when you look through a
microscope or stereoscope.
Body Tube: The cylindrical tube that holds the eyepiece.
Arm: The curved part that holds the body tube in place over the
stage.
Objectives: The lower cylindrical lenses on a microscope or
stereoscope that are closest to the specimen being viewed.
Magnification: Multiply the power of the eyepiece lens (10x) by the
power of the objective lens (40x) and you get the magnification (400x).
DIN Objectives: (Stands for Deutsche Industrie Normen) A DIN-threaded
lens is an international standard which insures compatibility
with lenses from any microscope manufacturer in the world.
Stage: The flat surface you put your slide or specimen on.
Stage Clips: The metal clips that hold the slide or specimen
stationary.
Mechanical (Moveable Stage): A device you attach to your
stage that holds the slide firmly and allows you to move it precisely
(most valuable at 400x or more where precision is critical).
Coarse Focus Knob: Used to roughly adjust the position of the body
tube, which quickly brings your specimen into focus.
Fine Focus Knob: Used to make adjustments to the body tube,
bringing your specimen into more fine focus. We highly recommend a fine
focus for most scientific study.
Slip Clutch: Keeps the stage from "slipping" so that you can focus
perfectly!
Parcentered: When the object in the center of your viewing area
stays centered when you rotate (change) objectives.
Parfocal: When the object in view stays in focus when you rotate
(change) objectives.
Iris Diaphragm: Working like the iris on an eye, this device is on
the underside of the stage. It adjusts (by opening and closing) the
amount of light that passes through the specimen.
Abbe Condenser: A device on the underside of the stage that
condenses and focuses the light coming through the diaphragm.
A moveable condenser is adjustable, whereas a non-moveable has a fixed
light focus. A condenser is most valuable at 1000x or higher.
Interpupillary Adjustment: The ability to adjust the spacing between
the two eyepieces on a binocular microscope so it fits a person's
interpupillary distance comfortably. The Seidentopf head allows for the best
adjustment by just twisting the eyepieces.
Oil Immersion: A lens that requires a drop of special oil on the
specimen for use. The oil is put on the cover slip, and the objective is
actually lowered into the oil. Oil immersion lenses are sealed so they
won't be damaged by the oil. Never lower a non-oil immersion lens into the
oil. Our Observer IV and Revelation lines both have a 100x oil immersion lens.
Illumination: The light source, either natural or artificial, at the
base of the microscope. All our illuminated microscopes are a cool
fluorescent or halogen type which is safest for your specimens. (Regular
tungsten lighting would create too much heat and kill some organisms!)
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