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Care & Usage

Proper Handling & Cleaning:

All of BestScopes.com's microscopes are fine instruments that, with good care and handling, will serve your needs for a lifetime! If children will be using our scopes, train them from the start in proper care and usage. This will give you the peace of mind to allow them the freedom to use the microscopes anytime, which will lead to greater scientific discoveries! Also bear in mind that practicing the old adage "out of sight, out of mind" may keep you and your children from using your microscope to its fullest potential. Keep it on a safe, sturdy surface where your family will most likely see it and use it. When not in use, keep the dust cover on it. Here are our suggestions for proper care and handling:

Remember...

  • Always lift your microscope with one hand holding the "arm" and the other hand under the base; avoid bumping or jarring the scope.
  • Use your microscope on a sturdy, solid surface (one that doesn't wobble) and away from the edge. (Countertops are great!)
  • Never touch the lenses of the eyepieces and objectives with your finger.
  • To keep your microscope in top condition for years, our manufacturer recommends that you have the microscope professionally serviced once a year.
  • Always keep the dust cover on your scope when it's not in use!
  • Keep all glass and optical lenses clean. Use lens paper or a Q-tip dipped in a lens cleaning solution (Windex works great!) to gently wipe dust/oils off the eyepieces, objectives, condenser, and illumination glass. (Never use a paper towel, tissue, or Kleenex to wipe a lens -- the fibers in them will scratch the lenses.)
  • Keep the stage and the other metal parts free of excess oil. An alcohol pad/wipe is best, but is not recommended for use on the lenses.

Proper Focusing:

  1. Always start with the lowest power objective in place over the hole in the stage.
  2. Using the coarse focus knob, raise the objective so it is one inch above the stage.
  3. While looking at your scope from the side, lower the objective with the coarse knob as close as possible to the specimen, without touching it.
  4. Using the same knob, focus upward while looking through the eyepieces, bringing specimen into focus.
  5. Now use your fine focus knob, if you have one, to clarify your specimen.
  6. You can rotate to the next highest objective, if needed.
  7. Always return to the lowest power objective before removing specimen.

Basic Slide Preparation

Temporary or Wet Mounts:

  1. Put drop of water in center of clean slide using eyedropper.
  2. Place specimen on top of water with tweezers.
  3. Hold one edge of coverslip so it touches the edge of the water drop.
  4. Carefully lower coverslip onto specimen, trying not to trap any air bubbles.
  5. Blot excess liquid outside of coverslip with paper towel.
  6. If slide starts to dry up, place a drop of water next to the edge of the coverslip that seems the most wet. Surface tension will pull the new drop of water under the coverslip.
  7. You can extend the life of your wet mount by sealing the edge of the cover slip with petroleum jelly.

Smear:

(Used to view cells in a liquid -- great for blood)
  1. Put specimen on one end of a clean slide.
  2. Using the edge of another clean slide, drag the edge across the liquid, creating a "smear" or thin layer.
  3. Gradually lower coverslip (one edge first) onto smear.
  4. Blot excess liquid with paper towel.

Pulling a stain:

  1. Prepare a wet mount.
  2. Put a drop of stain on edge of coverslip.
  3. Place a piece of tissue on the other edge of the coverslip opposite the drop of stain.
  4. The stain will be pulled under the coverslip. Add more stain a drop at a time until the stain reaches the tissue on the other end.
  5. Blot excess stain with paper towel.

Squash slide:

  1. Put drop of water on center of clean slide.
  2. Place a small piece of soft specimen on water drop.
  3. Place one edge of coverslip over specimen. Try not to trap any air bubbles.
  4. Place a piece of lens paper on top of coverslip and gently press to "squash" specimen.
  5. Blot excess liquid with paper towel.
 

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