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- Care and Usage
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Care & Usage
Proper Handling & Cleaning:
All of BestScopes.com's microscopes are fine instruments that, with good care and
handling, will serve your needs for a lifetime! If children will be using
our scopes, train them from the start in proper care and usage. This will
give you the peace of mind to allow them the freedom to use the microscopes
anytime, which will lead to greater scientific discoveries! Also bear in
mind that practicing the old adage "out of sight, out of mind" may keep you
and your children from using your microscope to its fullest potential. Keep
it on a safe, sturdy surface where your family will most likely see it and
use it. When not in use, keep the dust cover on it. Here are our
suggestions for proper care and handling:
Remember...
- Always lift your microscope with one hand holding the "arm" and the
other hand under the base; avoid bumping or jarring the scope.
- Use your microscope on a sturdy, solid surface (one that doesn't
wobble) and away from the edge. (Countertops are great!)
- Never touch the lenses of the eyepieces and objectives with your
finger.
- To keep your microscope in top condition for years, our manufacturer
recommends that you have the microscope professionally serviced once a
year.
- Always keep the dust cover on your scope when it's not in use!
- Keep all glass and optical lenses clean. Use lens paper or a Q-tip
dipped in a lens cleaning solution (Windex works great!) to gently wipe
dust/oils off the eyepieces, objectives, condenser, and illumination glass.
(Never use a paper towel, tissue, or Kleenex to wipe a lens --
the fibers in them will scratch the lenses.)
- Keep the stage and the other metal parts free of excess oil. An
alcohol pad/wipe is best, but is not recommended for use on the
lenses.
Proper Focusing:
- Always start with the lowest power objective in place over the hole in
the stage.
- Using the coarse focus knob, raise the objective so it is one inch
above the stage.
- While looking at your scope from the side, lower the objective
with the coarse knob as close as possible to the specimen, without
touching it.
- Using the same knob, focus upward while looking through the
eyepieces, bringing specimen into focus.
- Now use your fine focus knob, if you have one, to clarify your
specimen.
- You can rotate to the next highest objective, if needed.
- Always return to the lowest power objective before removing specimen.

Basic Slide Preparation
Temporary or Wet Mounts:
- Put drop of water in center of clean slide using eyedropper.
- Place specimen on top of water with tweezers.
- Hold one edge of coverslip so it touches the edge of the water drop.
- Carefully lower coverslip onto specimen, trying not to trap any air
bubbles.
- Blot excess liquid outside of coverslip with paper towel.
- If slide starts to dry up, place a drop of water next to the edge of
the coverslip that seems the most wet. Surface tension will pull
the new drop of water under the coverslip.
- You can extend the life of your wet mount by sealing the edge of the
cover slip with petroleum jelly.
Smear:
(Used to view cells in a liquid -- great for blood)
- Put specimen on one end of a clean slide.
- Using the edge of another clean slide, drag the edge across the
liquid, creating a "smear" or thin layer.
- Gradually lower coverslip (one edge first) onto smear.
- Blot excess liquid with paper towel.
Pulling a stain:
- Prepare a wet mount.
- Put a drop of stain on edge of coverslip.
- Place a piece of tissue on the other edge of the coverslip opposite
the drop of stain.
- The stain will be pulled under the coverslip. Add more stain a
drop at a time until the stain reaches the tissue on the other end.
- Blot excess stain with paper towel.
Squash slide:
- Put drop of water on center of clean slide.
- Place a small piece of soft specimen on water drop.
- Place one edge of coverslip over specimen. Try not to trap any air
bubbles.
- Place a piece of lens paper on top of coverslip and gently press to
"squash" specimen.
- Blot excess liquid with paper towel.
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